The Role of Bacillus pseudomycoides Isolates and Bioinformatics Modeling for Modern Pharmaceutical Applications: A Literature Review
DOI:
https://doi.org/10.38035/dhps.v2i3.1832Keywords:
Bacillus Pseudomycoides, Protease Enzymes, Bioinformatics, Pharmaceutical Applications, Molecular ModelingAbstract
This article presents a literature review on the role of Bacillus pseudomycoides isolates as protease enzyme producers and how bioinformatics modeling enhances their potential in modern pharmaceutical applications. B. pseudomycoides is known to produce proteases with superior characteristics such as temperature and pH stability, making them potential candidates for pharmaceutical formulations. Bioinformatics approaches such as protein structure modeling, molecular docking, and genomic analysis have accelerated the functional characterization of these enzymes. The combination of the utilization of microorganisms and computational approaches opens up new opportunities in drug development, wound therapy, and pharmaceutical biotechnology. This study recommends multidisciplinary integration in the utilization of microbial resources for future therapeutic needs.
References
Abdel-Fattah, Y. R., & Saeed, H. M. (2020). Cloning and expression of bacterial proteases for therapeutic applications. Biotechnology Advances, 44, 107611. https://doi.org/10.1016/j.biotechadv.2020.107611
Ahmad, T., Ahmad, R., & Alam, M. (2020). Production and characterization of alkaline protease from Bacillus pseudomycoides isolated from tannery effluent. Journal of Applied Biology and Biotechnology, 8(3), 72–78. https://doi.org/10.7324/JABB.2020.80312
Ananta, Y., Alamsjah, F., & Agustien, A. (2025). Optimization and molecular identification of PUA-14 bacterial isolate from protease-producing mangrove waters. Biodiversitas Journal of Biological Diversity, 26(1), 45-56. https://doi.org/10.1007/bio.2025.26.1.45
Chitte, R. R., Dey, S., & Sharma, A. (2020). Application of microbial proteases in wound healing: A holistic review. International Journal of Biological Macromolecules, 162, 1024–1038. https://doi.org/10.1016/j.ijbiomac.2020.06.066
Gupta, R., Beg, Q. K., & Lorenz, P. (2002). Bacterial alkaline proteases: Molecular approaches and industrial applications. Applied Microbiology and Biotechnology, 59(1), 15–32. https://doi.org/10.1007/s00253-002-0975-y
Hasan, F., Shah, A. A., & Hameed, A. (2019). Industrial applications of microbial proteases: A review. Enzyme and Microbial Technology, 39(2), 235–251. https://doi.org/10.1016/j.enzmictec.2005.10.016
Kumar, A., Jain, V. K., & Shanker, R. (2020). Optimization of protease production using response surface methodology from Bacillus species isolated from fermented food. Biotechnology Reports, 26, e00466. https://doi.org/10.1016/j.btre.2020.e00466
Liu, Y., Wang, W., & Chen, J. (2021). Comparative genomic insights into the protease genes in Bacillus species. Frontiers in Microbiology, 12, 631870. https://doi.org/10.3389/fmicb.2021.631870
Nguyen, T. T., Quyen, D. T., & Le, T. M. (2023). Advances in enzyme modeling and docking for pharmaceutical development. Biophysical Reviews, 15(1), 101–116. https://doi.org/10.1007/s12551-023-01018-2
Patel, S., & Goyal, A. (2019). Recent developments in enzymes for pharmaceutical applications. Current Enzyme Inhibition, 15(3), 174–183. https://doi.org/10.2174/1573408015666180406150002
Prajapati, R., & Patel, M. (2021). Therapeutic potential of microbial proteases in wound healing. Journal of Applied Microbiology, 130(4), 1043–1054. https://doi.org/10.1111/jam.15000
Rao, M. B., Tanksale, A. M., Ghatge, M. S., & Deshpande, V. V. (1998). Molecular and biotechnological aspects of microbial proteases. Microbiology and Molecular Biology Reviews, 62(3), 597–635. https://doi.org/10.1128/mmbr.62.3.597-635.1998
Sharma, S., Dangi, A. K., Shukla, P., & Kumar, A. (2022). In silico approaches in enzyme design: Current state and future prospects. Journal of Molecular Graphics and Modelling, 114, 108188. https://doi.org/10.1016/j.jmgm.2022.108188
Singh, R., Kumar, M., Mittal, A., & Mehta, P. K. (2020). Microbial enzymes: industrial progress in 21st century. 3 Biotech, 6(2), 174. https://doi.org/10.1007/s13205-016-0485-8
Zhou, L., Sun, J., Yu, M., & Yang, X. (2022). Enzyme engineering strategies for enhancing protease stability and activity. Applied Biochemistry and Biotechnology, 194(7), 3291–3307. https://doi.org/10.1007/s12010-022-04011-2
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Yorasakhi Ananta, Salsabila Dwi Fitri

This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright :
Authors who publish their manuscripts in this journal agree to the following conditions:
- Copyright in each article belongs to the author.
- The author acknowledges that the DHPS has the right to be the first to publish under a Creative Commons Attribution 4.0 International license (Attribution 4.0 International CC BY 4.0).
- Authors can submit articles separately, arrange the non-exclusive distribution of manuscripts that have been published in this journal to other versions (for example, sent to the author's institutional repository, publication in a book, etc.), by acknowledging that the manuscript has been published for the first time at DHPS.